Presence of a major (storage?) protein in dormant spores of the fungus Botryodiplodia theobromae.
نویسندگان
چکیده
Approximately 23% of the protein isolated from dormant spores of Botryodiplodia theobromae consisted of a single polypeptide; the polypeptide is probably degraded during germination.
منابع مشابه
In vitro translation of polyadenylate-containing RNAs from dormant and germinating spores of the fungus Botryodiplodia theobromae.
Polyadenylated RNA isolated by oligodeoxythymidylate-cellulose chromatography from spores of the fungus Botryodiplodia theobromae was translated in a cell-free protein-synthesizing system derived from wheat embryo. Reaction conditions which would yield efficient and accurate in vitro translation of the spore RNA were established. Dual isotopically labeled mixtures of in vitro translation produc...
متن کاملBiosynthesis and degradation of storage protein in spores of the fungus Botryodiplodia theobromae.
Muiridin, a spore-specific protein of the fungus Botryodiplodia theobromae, comprises about 25% of the mature pycnidiospore protein. It has an apparent molecular weight of 16,000 to 17,000 and is rich in glutamine, asparagine, and arginine. Muiridin is synthesized in developing spores via a precursor with an apparent molecular weight of 24,000. Two other polypeptides present in young developing...
متن کاملProtein synthesis during fungal spore germination: differential protein synthesis during germination of Botryodiplodia theobromae spores.
The preformed messenger ribonucleic acid in Botryodiplodia theobromae spores directs the synthesis of several relatively stable polypeptides
متن کاملProtein synthesis during fungal spore germination. II. Aminoacyl-soluble ribonucleic acid synthetase activities during germination of Botryodiplodia theobromae spores.
The specific activities of 13 aminoacyl-soluble ribonucleic acid (sRNA) synthetases were measured at various time intervals during the germination of Botryodiplodia theobromae conidiospores. The enzyme activities were low or absent in ungerminated spores, and they increased rapidly as germination proceeded. When extracts of the ungerminated spores were prepared with mortar and pestle, very litt...
متن کاملProtein synthesis during fungal spore germination. IV. Transfer ribonucleic acid from germinated and ungerminated spores.
Transfer ribonucleic acid (tRNA) fractions isolated from germinated and ungerminated spores of Botryodiplodia theobromae and Rhizopus stolonifer had acceptor activity for all 20 amino acids commonly found in protein, when tested with an enzyme fraction from germinated spores. Accordingly, it is unlikely that the absence of tRNA for a particular amino acid limits protein synthesis in fungal spores.
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عنوان ژورنال:
- Journal of bacteriology
دوره 138 2 شماره
صفحات -
تاریخ انتشار 1979